Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, Pv11статья

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Дата последнего поиска статьи во внешних источниках: 14 февраля 2019 г.

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[1] Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, pv11 / Y. Sogame, J. Okada, S. Kikuta et al. // Extremophiles. — 2017. — Vol. 21, no. 1. — P. 65–72. Larvae of the African midge Polypedilum vanderplanki show extreme desiccation tolerance, known as anhydrobiosis. Recently, the cultured cell line Pv11 was derived from this species; Pv11 cells can be preserved in the dry state for over 6 months and retain their proliferation potential. Here, we attempted to expand the use of Pv11 cells as a model to investigate the mechanisms underlying anhydrobiosis in P. vanderplanki. A newly developed vector comprising a constitutive promoter for the PvGapdh gene allowed the expression of exogenous proteins in Pv11 cells. Using this vector, a stable Pv11 cell line expressing green fluorescence protein (GFP) was established and retained desiccation tolerance. Gene silencing with GFP-specific siRNAs significantly suppressed GFP expression to approximately 7.5-34.6% of that in the non-siRNA-transfected GFP stable line. Establishment of these functional assays will enable Pv11 cells to be utilized as an effective tool to investigate the molecular mechanisms underlying anhydrobiosis.

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