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Phosphorylation of troponin T by Ca-phospholipid-dependent protein kinaseстатья
Информация о цитировании статьи получена из
Web of Science
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Risnik V.V., Vorotnikov A.V., Gusev N.B.
- Журнал: Biomedica biochimica acta
- Том: 46
- Номер: 8-9
- Год издания: 1987
- Издательство: Akademie Verlag
- Местоположение издательства: Germany
- Первая страница: 444
- Аннотация: Phosphorylation of skeletal and cardiac troponin T by Ca-phospholipid-dependent protein kinase/protein kinase C/was investigated. Under conditions used, the rate of troponin T phosphorylation was only 3-4 times lower than that of histone H-1, and after long term incubation the enzyme incorporated about 1 mol of phosphate per mol of skeletal and cardiac troponin T. The sites phosphorylated by protein kinase C are mainly located in the C-terminal part/residues 159-259/of skeletal troponin T. The less effectively phosphorylated sites are located in between residues 42-158 of troponin T. Reverse phase HPLC revealed four groups of tryptic phosphopeptides of troponin T. The phosphopeptides weakly adsorbed on a RP-18 column were identified as a mixture of two peptides/GKKQTAR and QTAR/both containing Thr-171 of troponin T phosphorylated by protein kinase C.
- Добавил в систему: Гусев Николай Борисович