Аннотация:The segregation of absorption sites of cationic dye (acridin orange) and anionic dye (uranin) has been demonstrated in Elodea canadensis Minch by flourescent method. The dyes were absorbed both by roots and by leaves of Elodea canadensis Minch. Segregation of the uptake of cationic and anionic dyes was demonstrated in special transparent cells - hydropots that are in leaves. Some of these cells absorbed acridin orange, and other ones - uranin. Proportion of hydropots decreased with the leaf's age. In root hair cells the cationic dye was uptaken by the tip of a root hair, and anionic dye was uptaken by the basic part of a cell which is in the risodermis. In the roots without root hair cells cationic dye was absorbed by single cells near the root base and anionic dye was absorbed by single cells near the root apex.