Multi-Wavelength Thermal-Lens Spectrometry for High-Accuracy Measurements of Absorptivities and Quantum Yield of Photodegradation of a Hemoprotein-Lipid Complexстатья

Статья опубликована в высокорейтинговом журнале

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Дата последнего поиска статьи во внешних источниках: 17 октября 2017 г.

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[1] Multi-wavelength thermal-lens spectrometry for high-accuracy measurements of absorptivities and quantum yield of photodegradation of a hemoprotein-lipid complex / K. Tishchenko, M. Muratova, D. Volkov et al. // Arabian Journal of Chemistry. — 2017. — Vol. 10, no. 6. — P. 781–791. Accurate measurements of absorptivities and quantum yields of biochemical species under various conditions are an important task of applied photonics and analytical chemistry. In this work, we provide a comparison of the capabilities of thermal-lens spectrometry to measure these parameters of various samples. Measurements of relevant model substances, biologically active substituted 2-thiohydantoins and their complexes of copper(I,II) and heme proteins (forms of hemoglobin and cytochrome c), showed negligible differences in apparent molar absorptivities for thermal-lens spectrometry and optical-absorption (spectrophotometric) data. The values for tabletop and microscale thermal-lens measurements under batch conditions differ insignificantly. The precision of measurements of molar absorptivities by thermal-lens spectrometry is no less than in the case of spectrophotometry or the precision is even higher in the cases of low absorptivities. For cardiolipin–cytochrome c-NO complex, the difference between absorptivity values calculated from thermal-lens data and acquired by spectrophotometry is significant due to complex photodegradation. The quantum yield of its photolysis reaction calculated from optical absorption and thermal-lens data altogether at two wavelengths for 0.4–360 s of the reaction, 0.46 ± 0.04, was estimated. Keywords Multispectral photothermal spectroscopy; Thermal-lens spectrometry; 2-thiohydantoins (2-thioxo-imidazolidin-4-ones); Cytochrome c; Hemoglobin; Absorptivity. [ DOI ]

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