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A NEW WAY IN HOMOGENEOUS IMMUNOASSAY - REVERSED MICELLAR SYSTEMS AS A MEDIUM FOR ANALYSISстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: KABANOV AV, KHRUTSKAYA MM, EREMIN SA, KLYACHKO NL, LEVASHOV AV
- Журнал: Analytical Biochemistry
- Том: 181
- Номер: 1
- Год издания: 1989
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 145
- Последняя страница: 148
- DOI: 10.1016/0003-2697(89)90408-9
- Аннотация: Possibilities of a new principle for the homogeneous enzyme immunoassay utilizing the systems of surfactant reversed micelles in organic solvents have been demonstrated taking thyroxine determination as an example. The catalytic activity of an enzyme, solubilized in such systems, is determined by the ratio of geometric dimensions of the micellar matrice and the enzyme molecule. The addition of antibodies against thyroxine to the peroxidase-thyroxine conjugate, solubilized in the system of reversed micelles of aerosol OT in octane, leads to the formation of the immune complex whose size differs substantially from that of the initial enzyme-antigen conjugate. This induces changes in the peroxidase catalytic activity. The addition of free thyroxine to the system stimulates the conjugate release from the immune complex and, consequently, the reduction of the peroxidase activity to the initial level. Sensitivity of the analysis in reversed micellar systems can be regulated by changing the surfactant hydration degree. Substances of different nature (both hydrophobic and hydrophylic) can be solubilized in reversed micellar systems under standard conditions, which allows determination of water insoluble antigens.
- Добавил в систему: Клячко Наталья Львовна