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NEW IMMUNOASSAY TECHNIQUE USING ANTIBODY IMMOBILIZED ON A MEMBRANE AND A FLOW CUVETTE AS REACTION VESSELстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: GOROVITS BM, OSIPOV AP, EGOROV AM
- Журнал: Journal of Immunological Methods
- Том: 157
- Номер: 1-2
- Год издания: 1993
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 11
- Последняя страница: 17
- DOI: 10.1016/0022-1759(93)90064-E
- Аннотация: Immunoenzymatic detection systems have been developed using human IgG as a model antigen. A membrane with covalently immobilized specific antibodies was placed into a specially constructed ultranarrow flow cuvette and solutions containing the antigen and antibody-peroxidase conjugate were then successively passed through the flow capillary cell. After washing, the membrane was placed into the substrate solution and the intensity of developed colour on the membrane was recorded visually or by a reflection spectrophotometer. The lower detection limit was about 5 x 10(-11) M and the overall analysis time was 10 min. Photoimmobilization was used to immobilize the antibody and thereby permitting control of the protein surface concentration on the membrane as well as the dimensions and shape of the activated region.
- Добавил в систему: Осипов Александр Павлович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | New_immunoassay_technique__using_antibody_immobilized_on_... | New_immunoassay_technique__using_antibody_immobilized_on_... | 1,9 МБ | 5 февраля 2014 [APOsipov] |
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| [1] | GOROVITS B., OSIPOV A., EGOROV A. New immunoassay technique using antibody immobilized on a membrane and a flow cuvette as reaction vessel // Journal of Immunological Methods. — 1993. — Vol. 157, no. 1-2. — P. 11–17. Immunoenzymatic detection systems have been developed using human IgG as a model antigen. A membrane with covalently immobilized specific antibodies was placed into a specially constructed ultranarrow flow cuvette and solutions containing the antigen and antibody-peroxidase conjugate were then successively passed through the flow capillary cell. After washing, the membrane was placed into the substrate solution and the intensity of developed colour on the membrane was recorded visually or by a reflection spectrophotometer. The lower detection limit was about 5 x 10(-11) M and the overall analysis time was 10 min. Photoimmobilization was used to immobilize the antibody and thereby permitting control of the protein surface concentration on the membrane as well as the dimensions and shape of the activated region. [ DOI ] |