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Simultaneous determination of sulphamerazine, sulphamethazine and sulphadiazine in honey and chicken muscle by a new monoclonal antibody-based fluorescence polarisation immunoassayстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Wang Zhanhui, Zhang Suxia, Ding Shuangyang, Eremin Sergei A., Shen Jianzhong
- Журнал: Food additives and contaminants
- Том: 25
- Номер: 5
- Год издания: 2008
- Издательство: Taylor & Francis
- Местоположение издательства: United Kingdom
- Первая страница: 574
- Последняя страница: 582
- DOI: 10.1080/02652030701713913
- Аннотация: A new monoclonal antibody (Mab) against sulphamerazine (SMR) was produced and a fluorescence polarisation immunoassay (FPIA) based on the Mab was developed and optimized for the simultaneous qualitative screening of SMR, sulphamethazine (SMZ) and sulphadiazine (SDZ). The Mab, raised from mice immunized with SMR, was bound to bovine serum albumin (BSA) using glutaraldehyde as the coupling reagent. Fluorescein-labelled SMR and SMZ (tracer) were synthesized and purified by thin layer chromatography (TLC). Cross-reactivities below 3.6% were displayed in the optimized FPIA for another 14 sulphonamides when both tracers were employed. The limits of detection (LOD) were 0.9 ng g(-1) for SMR, 2 ng g(-1) for SMZ and 3.1 ng g(-1) for SDZ. Analysis of SMR, SMZ and SDZ fortified chicken muscle and honey samples by the FPIA showed average recoveries of 86-131% with a standard deviation (SD) of 4.6-32. Comparative analyses of a SMZ-treated chicken muscle sample by both FPIA and high performance liquid chromatograph (HPLC) showed a good correlation (r = 0. 999 1). The study demonstrates the practical application of FPIA in screening chicken muscle and honey samples for sulphonamides residues.
- Добавил в систему: Еремин Сергей Александрович