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Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensorsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 декабря 2018 г.
- Авторы: Tarrago Lionel, Péterfi Zalán, Lee Byung Cheon, Michel Thomas, Gladyshev Vadim N.
- Журнал: Nature Chemical Biology
- Том: 11
- Номер: 5
- Год издания: 2015
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 332
- Последняя страница: 338
- DOI: 10.1038/nchembio.1787
- Аннотация: Methionine can be reversibly oxidized to methionine sulfoxide (MetO) under physiological and pathophysiological conditions, but its use as a redox marker suffers from the lack of tools to detect and quantify MetO within cells. In this work, we created a pair of complementary stereospecific genetically encoded mechanism-based ratiometric fluorescent sensors of MetO by inserting a circularly permuted yellow fluorescent protein between yeast methionine sulfoxide reductases and thioredoxins. The two sensors, respectively named MetSOx and MetROx for their ability to detect S and R forms of MetO, were used for targeted analysis of protein oxidation, regulation and repair as well as for monitoring MetO in bacterial and mammalian cells, analyzing compartment-specific changes in MetO and examining responses to physiological stimuli.
- Добавил в систему: Гладышев Вадим Николаевич