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Topical treatment with the bacterium-derived c-met agonist InlB321/15 accelerates healing in the abrasion wound mouse modelстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 декабря 2018 г.
- Авторы: Chalenko Y.M., Sysolyatina E.V., Sobyanin K.A., Kapkaeva M.R., Lavrikova A., Kalinin E., Scheglovitova O.N., Ermolaeva S.A.
- Журнал: Archives of Dermatological Research
- Том: 310
- Год издания: 2018
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 849
- Последняя страница: 856
- DOI: 10.1007/s00403-018-1870-4.
- Аннотация: Studies of factors affecting wound-healing rates are encouraged by a critical need for new treatments to manage an increasing burden of non-healing wounds. The InlB protein produced by the Gram-positive bacterium Listeria monocytogenes is an agonist of the tyrosine kinase receptor c-Met and a functional analog of the hepatocyte growth factor (HGF), which is a mammalian ligand of c-Met. The recombinant InlB321 protein, which is the c-Met-binding InlB domain (amino acids 31-321), was cloned from the L. monocytogenes serovar 4b clinical strain VIMHA015 and serovar 1/2a strain EGDe (InlB321/15 and InlB321/EGDe, respectively). Both InlB321 variants stimulated proliferation of endothelial HUVEC cells. InlB321/15 was more active in Erk1/2 phosphorylation assay, and more potent than InlB321/EGDe in the 2D-scratch wound-healing assay. Scratch closure reached 86%, 29% and 72% for InlB321/15, InlB321/EGDe and HGF, respectively, 72 h post-wounding (p < 0.05). Topically applied glycerol-mixed InlB321/15 (300 µg ml- 1) increased abrasion wound-healing rates in mice. The 50% wound closing time (CT50) was reduced by InlB321/15 (4.18 ± 0.91 days; CI: 3.05; 5.31) compared with control animals (5.51 ± 1.21 days; CI: 4.01; 7.01; p < 0.05). Taken together, obtained results suggested a potential of InlB321/15 as a means of accelerating wound healing. KEYWORDS: Cutaneous wounds; Hepatocyte growth factor; InlB; Wound healing; c-Met
- Добавил в систему: Капкаева Марина Рафаилевна