New Approaches to Cognitive Neurobiology: Methods for Two-Photon in Vivo Imaging of Cognitively Active Neuronsстатья
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Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 апреля 2019 г.
Авторы:
Marina Roshchina ,
Olga Ivashkina ,
Anokhin Konstantin V.
Журнал:
Neuroscience and Behavioral Physiology
Том:
48
Номер:
5
Год издания:
2018
Издательство:
Kluwer Academic/Plenum Publishers
Местоположение издательства:
United States
Первая страница:
741
Последняя страница:
746
DOI:
10.1007/s11055-018-0625-1
Аннотация:
This review addresses the potential of current methods of in vivo two-photon imaging of the activity of neurons involved in episodes of cognitive activity in animals. The principles of fluorescent two-photon microscopy are described and methods for in vivo imaging of neuron activity using calcium indicators of two types – calcium stains and genetically encoded calcium indictors (GECI) – are discussed. A new approach is also considered, using in vivo imaging of genomic activation of cerebral neurons in transgenic animals with fluorescent probes for the expression of the immediate early genes c-fos, Arc, and Egr-1. The main advantages and disadvantages of these approaches are compared and the potentials for the development of in vivo two-photon imaging of neuron activity for studies of the cellular basis of higher brain functions are addressed. © 2018 Springer Science+Business Media, LLC, part of Springer Nature
Добавил в систему:
Анохин Константин Владимирович