Monomolecular enzyme films stabilized by amphiphilic polyelectrolytes for biosensor devicesстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Авторы:
Eremenko A. ,
Kurochkin I. ,
Chernov S. ,
Barmin A. ,
Yaroslavov A. ,
Moskvitina T.
Журнал:
Thin Solid Films
Том:
260
Номер:
2
Год издания:
1995
Издательство:
Elsevier Sequoia
Местоположение издательства:
Switzerland
Первая страница:
212
Последняя страница:
216
DOI:
10.1016/0040-6090(94)06403-2
Аннотация:
This paper describes the use of new amphiphilic polyelectrolytes for protein immobilization. Monomolecular films of glucose oxidase (GO) and monoamine oxidase (MAO) stabilized by amphiphilic polyelectrolytes (polyethyleneimine and poly-4-vinylpyridine derivatives modified by lauryl chains) were formed on a water surface in a Langmuir-Blodgett trough. The compressed films containing the enzymes were transferred onto the surface of a polypropylene membrane of a Clark electrode according to the Langmuir-Schaefer method. The analytical responses of the resulting biosensors were linear over the range 1-10 mM of glucose and 8-100 μM of tyramine. Furthermore, direct functional coupling of GO and ferrocenecarboxylic acid in multilayer films stabilized by amphiphilic polyelectrolytes was demonstrated. The amperometric response of such a sensor was linear over the range 1-20 mM of glucose. The dependence of the kinetic parameters of the enzymes on the amphiphilic polyelectrolyte structures is discussed. © 1995.
Добавил в систему:
Курочкин Илья Николаевич