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Induction of multipolar mitoses in cultured cells: decay and restructuring of the mitotic apparatus and distribution of centriolesстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Alieva I.B., Vorobjev I.A.
- Журнал: Chromosoma
- Том: 100
- Номер: 8
- Год издания: 1991
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 532
- Последняя страница: 542
- Аннотация: During recovery after a long (up to 12 h) treatment of pig embryo culture cells (PK) with nocodazole at concentrations of 0.02 microgram/ml and 0.2 microgram/ml all c-metaphase cells divide normally into two daughter cells. During recovery after a short (1-4 h) treatment with 0.6 microgram/ml nocodazole only multipolar mitoses (as a rule tripolar) arise. At the ultrastructural level, the increasing nocodazole concentration leads to progressive disruption of the mitotic spindle. At a nocodazole concentration of 0.2 microgram/ml kinetochores are not associated with microtubules. At a nocodazole concentration of 0.6 microgram/ml there are no microtubules around the centrosomes, and in every cell one of the two diplosomes disintegrates. In tripolar telophase centrioles are distributed among the spindle poles generally in a 2:2:0 pattern. Mother and daughter centrioles are always disoriented but not separated. The centriole-free pole contains a cloud of electron-dense material. During tripolar division two of the three daughter cells mainly fuse shortly after telophase forming one binucleate cell. Thus a multipolar mitosis arises as a result of the uncoupling of mother centrioles and spindle microtubules, but not of the duration of the c-mitotic arrest. Centriole-free poles account for the divergence of chromosomes, but mainly they are unable to ensure the normal cytokinesis of daughter cells.
- Добавил в систему: Алиева Ирина Борисовна