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SYNTHETIC DNA DUPLEXES AS A TOOL IN STUDYING THE MECHANISM OF ECORII ACTIVATIONстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Petrauskene O.V., Kubareva E.A., Gromova E.S., Pein C.D., Cech D., Shabarova Z.A.
- Журнал: Molecular Biology
- Том: 27
- Номер: 3
- Год издания: 1993
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 301
- Последняя страница: 307
- Аннотация: The efficiency of EcoRII cleavage of synthetic DNA duplexes with one EcoRII recognition site decreases with increasing substrate length. This enzyme virtually fails to cleave DNA duplexes longer than 215 bp. However, EcoRII cleaves long DNA duplexes with one recognition site in the presence of 11-14-bp substrates. The extent of hydrolysis activation depends on the length and concentration of the added substrate. A model system is suggested for studying the molecular and kinetic mechanism of EcoRII activation. This system includes a 30-bp substrate with one EcoRII recognition site, and DNA duplexes as activating substrates that contain modified heterocyclic bases and internucleotide phosphate groups. DNA duplexes with a modified EcoRII recognition site may activate hydrolysis of the 30-bp substrate and of phage T3 DNA. Their catalytic effect on the cleavage of extended duplexes depends on the type of modification and its localization in the recognition site. Cooperative interaction of EcoRII with two recognition sites in DNA has been shown to be essential for the functioning of the enzyme.
- Добавил в систему: Кубарева Елена Александровна