The potentiometric biosensor based on polyaniline semiconductor filmsстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:A potentiometric biosensor based on semiconductor polyaniline is developed. The potentiometric response of the polyaniline films to a change in pH is 65-70 mV per pH unit, which is higher than that of the known pH transducers. The polyaniline films prepared by electropolymerization contained immobilized trypsin as a model enzyme. The high potentiometric response of the polyaniline films and the incorporation of enzyme into the pH-sensitive matrix ensured high sensitivity of the biosensor. The potentiometric electrode thus produced can be used for analyzing substrate concentrations ranging from 5 x 10(-5) to 1 x 10(-2) M. Th, time response does not exceed 20-40 s. The maximum potentiometric response of the electrode reaches 270 mV in a 0.001 M phosphate buffer.