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Cylindrical substratum induces different patterns of actin microfilament bundles in nontransformed and in ras-transformed epitheliocytesстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Levina E.M.a, Domnina L.V.b, Rovensky Y.A.a.c, Vasiliev J.M.a
- Журнал: Experimental Cell Research
- Том: 229
- Номер: 1
- Год издания: 1996
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 159
- Последняя страница: 165
- DOI: 10.1006/excr.1996.0354
- Аннотация: The influence of the cylindrical substratum surface on the actin micro filament bundle and the extracellular matrix (fibronectin and laminin) patterns in nontransformed epithelial cells of the IAR-2 rat line and in their N-ras-transformed descendants, IAR-ras-c4 cells, was studied. The cells were cultured on substrata with cylindrical surfaces-fused quartz glass fibers with a diameter of 32 μm. Quantitative analysis of actin microfilament bundle alignment and immunomorphological study of fibronectin and laminin were used. IAR-2 epitheliocytes on the cylindrical substrata formed straight actin microfilament bundles and fibronectin- or laminin- positive fibrils aligned predominantly transversely to the cylinder axis. In contrast, in the majority of IAR-ras-c4 cells on the cylindrical substrata, the revealed straight micro filament bundles and the fibrils of the extracellular matrix were oriented approximately longitudinally to the cylinder axis; a small part of the transformed cells formed microfilament bundles and extracellular matrix patterns similar to those in the normal epitheliocytes on the cylindrical substrata. These results show that transformed epitheliocytes that acquired polarized morphology react to the curvature of the cylindrical substratum surface by actin cytoskeleton and extracellular matrix reorganization changes essentially different from those characteristic of normal discoid epitheliocytes on the cylindrical substrata, but similar to those observed in normal polarized cells, e.g., fibroblasts.
- Добавил в систему: Домнина Лидия Владимировна