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Chlorophyll a Phytylation is Required for the Stability of Photosystems I and II in the Cyanobacterium Synechocystis sp. PCC 6803статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или ScopusДата последнего поиска статьи во внешних источниках: 9 декабря 2012 г.
- Авторы: Shpilyov A.V., Zinchenko V.V., Grimm B., Lokstein H.
- Журнал: Plant Journal
- Год издания: 2012
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- DOI: 10.1111/tpj.12044
- Аннотация: In oxygenic phototrophic organisms, the phytyl ’tail’ of chlorophyll a is formed from a geranylgeranyl residue by the enzyme geranylgeranyl reductase. Additionally, in oxygenic phototrophs phytyl residues are the tail moieties of tocopherols and phylloquinone. A mutant of the cyanobacterium Synechocystis sp. PCC 6803 lacking geranylgeranyl reductase, ΔchlP, was compared to strains with specific deficiencies in either tocopherols or phylloquinone to assess the role of chlorophyll a phytylatation (vs. geranylgeranylation). The tocopherol-less Δhpt strain grows indistinguishably from the wild type under ’standard’ light photoautotrophic conditions and exhibited only a slightly enhanced rate of photosystem I degradation under strong irradiation. The phylloquinone-less ΔmenA mutant also grows photoautotrophically, albeit rather slowly and only at low light intensities. Under strong irradiation, ΔmenA retained its chlorophyll content indicative of stable photosystems. Discernibly, ΔchlP can be cultured only photomixotrophically (due to instability of both, photosystems I and II). Increased accumulation of myxoxanthophyll in ΔchlP cells indicates photo-oxidative stress even under moderate illumination. Under high light conditions, ΔchlP exhibited rapidly degrading photosystems I and II. In conclusion, the results demonstrate that chlorophyll a phytylation is important for (photo)stability of photosystems I and II, which, in turn, is necessary for photoautotrophic growth and tolerance of high light in an oxygenic environment. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
- Добавил в систему: Зинченко Владислав Владимирович