Influence of a single modification of the DNA sugar-phosphate backbone on the functioning of restriction endonucleases EcoRII, MvaI, and BstN1статья
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Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
Аннотация:Oligonucleotide duplexes containing modified nucleoside residues 1-(2'-deoxy-B-D-threo-pentofuranosyl)thymine (xT) or 1-(3'-deoxy-B-D-threo-pentofuranosyl)uracil (tU) in the center of the recognition site for EcoRII, MvaI, and BstN1 (CCA/(T)GG) were constructed and examined in respect of their physicochemical and substrate properties. The restriction endonucleases proved to differ in their sensitivity to the resulting conformational distortions. For the first time with EcoRII, the xT-containing analog turned out to be a much better substrate and, moreover, an activator of the enzyme.