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Chemical cross-linking of MvaI and EcoRII enzymes to DNA duplexes containing a monosubstituted pyrophosphate internucleotide bondстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Sheflyan G.Ya, Kubareva E.A., Volkov E.M., Oretskaya T.S., Gromova E.S., Shabarova Z.A.
- Журнал: Gene
- Том: 157
- Номер: 1,2
- Год издания: 1995
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 187
- Последняя страница: 190
- DOI: 10.1016/0378-1119(94)00729-C
- Аннотация: DNA duplexes containing a monosubstituted pyrophosphate internucleotide group, instead of a phosphodiester bond, were used as cross-linking reagent for the affinity modification of the restriction endonucleases EcoRII and MvaI (R . EcoRII and R . MvaI). An active group was introduced into the enzyme's recognition site or between the recognition site and flanking sequence. The substrate properties of such DNA duplexes were determined. Cross-linking specificity was demonstrated by competition experiments with unmodified substrate, as well as by the absence of cross-linking to an active duplex lacking a recognition site. It was shown that the nucleophilicity of the buffer solution and the presence of the enzyme cofactor Mg2+ dramatically affected the cross-linking yield.
- Добавил в систему: Орецкая Татьяна Семеновна