From an increase in the number of tandem repeats through the decrease of sialylation to the down-regulation of MUC1 expression levelстатья Исследовательская статья

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Дата последнего поиска статьи во внешних источниках: 19 марта 2019 г.

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[1] From an increase in the number of tandem repeats through the decrease of sialylation to the down-regulation of muc1 expression level / M. S. Syrkinaa, V. S. Viushkov, D. M. Potashnikova et al. // Journal of Cellular Biochemistry. — 2019. — Vol. 120, no. 3. — P. 4472–4484. Enhanced glucose uptake by cancer cells was demonstrated in many studies in vitro and in vivo. Glycolysis is one of the main ways of obtaining energy in the hypoxia conditions. But in addition to energy exchange carbohydrates are necessary for the post-translational modification of the protein molecules. Cancer cells are often characterized by the enhanced expression of different glycoproteides. Correct glycosylation defines the structure and activity of such molecules. We demonstrated that under the same cultivation conditions the intensity of glycosylation does not depend on the total number of potential O-glycosylation sites in one molecule. As a model for the investigation the tandem repeat region (VNTR) of the human mucin MUC1, in which each of the repeats carries 4 potential O-glycosylation sites, was used. An increase of the tandem repeat number in the recombinant protein did not lead to a proportional increase in the level of sLea glycosides. A consequence of this was a reduction in the number of recombinant proteins associated with the cytoplasmic membrane at a overall high expression level. Prolongation of the cultivation duration leds to a reduction in the expression level of the recombinant proteins up to 30 % of the initial level and the intensity of such reduction was in a direct ratio of the number of tandem repeats in the protein molecule. [ DOI ]

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