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IL-2-induced NF-κB phosphorylation upregulates cation nonselective conductance in human cardiac fibroblastsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 ноября 2018 г.
- Авторы: Mitrokhin V., Gorbacheva L., Mladenov M., Kamkin A.
- Журнал: International Immunopharmacology
- Том: 3
- Номер: 64
- Год издания: 2018
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 170
- Последняя страница: 174
- DOI: 10.1016/j.intimp.2018.08.040
- Аннотация: PURPOSE: Studies of negative ionotropic effects of IL-2 create the basis for possible IL-2 impact on nonselective conductance (GNS), which potentially makes these effects useful in elucidation of the pathways affected by IL-2. MATERIALS AND METHODS: A culture of human cardiac fibroblasts (CHCFs) was used in this study. A voltage clamp mode of the whole-cell patch-clamp technique was introduced. The level of phosphorylated NF-κB was determined by newly developed semi-quantitative ELISA. RESULTS: The IL-2 (5 ng/ml) increased the currents during the depolarizing clamp to larger amplitudes without changing their time course. In the CHCFs pretreated with 50 μmol/L 2-APB, IL-2-induced increase in GNS was highly prevented (p < 0.001), indicating possible STIM-ORAI involvement. The CHCF perfusion with IL-2 in the presence of IMD-0354 for 14-16 min confirmed a significant GNS prevention (between 50 and 80%), indicating IκB involvement in the IL-2-induced signaling. The CHCF perfusion with IL-2 in the presence of Chel, induced significant prevention in the GNS expression (between 50 and 80%) compared to IL-2 treated cells, indicating PKC involvement. CONCLUSIONS: IL-2 mediated GNS increase is mediated by activation of downstream players such as PKC, IκB, and NF-κB, which are probably further responsible for the upregulation of STIM-ORAI.
- Добавил в систему: Горбачева Любовь Руфэльевна