Method for Ultrarapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen

Авторы: Roman Chuprov-Netochin, Elena Marusich, Yaroslav Neskorodov, Yana Mishutkina, Polina Volynchuk, Yan Ivanenkov, Alisher Touraev, Sergey Leonov, Klaus Palme
Сборник: Plant Chemical Genomics
Серия: Methods and Protocols
Глава в коллективной монографии
Год издания: 2018
Место издания: Humana Press New York, NY
Первая страница: 27
Последняя страница: 37
DOI: 10.1007/978-1-4939-7874-8_3
Аннотация: Chemical genomics attracts considerable attention by offering crucial tools for plant biology to regulate plant growth and development. However, most chemical screens are time consuming and laborious, and require a high input of resources. Here we describe a broadly applicable method for the ultrarapid high-content phenotypic screening of small chemical compound libraries for new plant growth regulators. The assay is based on determination of pollen tube growth and can be completed in less than 8 h. Using this method, we identified novel inhibitors and modulators of plant growth and showed that compounds selected using a Nicotiana tabacum-based assay were biologically active in other plants, such as Arabidopsis thaliana.
Добавил в систему: Нескородов Ярослав Борисович

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