Effect of various additives on the processes of aggregation and dissociation of coat proteins of several helical plant viruses

Ksenofontov, A.L.; Dobrov, E.N.; Ksenofontova, O.B.; Deigin, V.I.; Shtykova, E.V.

Авторы: Ksenofontov A.L., Dobrov E.N., Ksenofontova O.B., Deigin V.I., Shtykova E.V.
Сборник: FEBS Open Bio
Серия: Supplement 1
Том: 8
Тезисы
Год издания: 2018
Место издания: FEBS Open Bio Prague, Czech Republic
Первая страница: 432
Последняя страница: 432
DOI: 10.1002/2211-5463.12453
Аннотация: Factors determining the direction of aggregation and dissociationprocesses of the coat protein (CP) of several helical plant viruseshave been studied. The ability of various additives (polyelec-trolyte’s, surfactants and same peptides) to prevent protein aggre-gation was analyzed. We have shown that the CP of potyvirus –Potato Virus A (PVA) – forms associates – clusters consisted of30 subunits of CP’s, that retain in water-salt solution withincreased ionic strength and pH (pH 10.5, 0.5 M NaCl). To ana-lyze a low-resolution structure of the protein in solution, we useda method of Small-Angle X-ray Scattering. It was shown thatparticles of CP PVA do not dissociate under the influence of10 mM dextran sulfates (15, 100 kDa) but dissociate under theaction of 5 mM sodium dodecyl sulfate (SDS). We observed for-mation complexes of “CP-SDS”, consisting of 10–12 small parti-cles joined together as “necklace and bead” complex. The similareffect of SDS is shown for serum albumins (BSA, HSA). Spectralmethods show the preservation of secondary structure and lossof tertiary one of the complexes of CP PVA -SDS. The effect ofadditives on the process of aggregation was studied in the modelof amorphous aggregation CP’s of potyvirus PVA and to-bamovirus TMV, induced by heating to 42C. We tested speciallyselected neuropeptides (5–7 aa) and immunomodulatory peptide(2–3 aa). The kinetics of aggregation was monitored by UVabsorption at 320 nm by UV-2600 Spectrophotometer andDynamic Light Scattering by Malvern ZetaSizer instrument.Among some tested peptides, several peptides were effective insuppressing aggregation; others accelerated this process. The pos-sible mechanism and role of disordered segments of CP’s plantviruses in the processes their aggregation and dissociation are dis-cussed. The work was supported by the Russian Foundation forBasic Research (projects 18-04-00525) and by the Federal Agencyof Scientific Organizations (Agreement N 007-ГЗ/Ч3363/26) inpart of SAXS.
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