[2'fluoro derivatives of nucleosides as substrates of viral replicative nucleotide polymerases]статья

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Дата последнего поиска статьи во внешних источниках: 20 апреля 2016 г.

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[1] [2'fluoro derivatives of nucleosides as substrates of viral replicative nucleotide polymerases] / M. K. Kukhanova, A. N. Korovina, I. A. Sharkin et al. // Molekuliarnaia biologiia. — 2014. — Vol. 48, no. 5. — P. 834–841. Substrate specificities of three viral replicative polymerases of different origins (HIV reverse transcriptase, hepatitis C virus RNA polymerase, and herpes virus DNA polymerase) towards 2'F-NTP were studied. Activated DNA, polyA-oligoUs and (2'F-A)20-oligoU6-complexes were used as templates. It was shown that all DNA polymerases studied can incorporate 2'F-NMP into the 3'-end of primer-template complexes. HIV reverse transcriptase and herpes virus DNA polymerase can elongate synthesis with both dNTP and 2'F-NTP. Homopolymer (2'F-A)20 can serve as a template for polymerization of both UTP and 2'F-UTP,-catalyzed by hepatitis C virus polymerase although with efficacy about 5 to 10-fold lower in comparison with natural primertemplate complex. Pyrophosphorolysis reaction of 2'F-CMP residue at 3'-end of primer catalyzed with HIV reverse transcriptase is going by two orders of magnitude less effective if compared with natural dNMP residue at the same system.

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