The possibilities of using fungal fluorophores to assess responses of filamentous fungi to external stimuliтезисы доклада

Дата последнего поиска статьи во внешних источниках: 16 января 2019 г.

Работа с тезисами доклада


[1] The possibilities of using fungal fluorophores to assess responses of filamentous fungi to external stimuli / E. Fedoseeva, D. Khundzhua, V. Terekhova et al. // SETAC Europe 28th Annual Meeting. — Abstract Book. — SETAC Europe Rome, 2018. — P. 532–532. 532 The possibilities of using fungal fluorophores to assess responses of filamentous fungi to external stimuli E.V. Fedoseeva, Pirogov Russian National Research Medical University / Pediatric faculty; D. Khundzhua, Lomonosov Moscow State University / Depament of General Physics; V. Terekhova, Lomonosov Moscow State University / Lab of Ecotoxicological Soil Analysis; M. Freidkin, Lomonosov Moscow State University / Department of General Physics; S. Patsaeva, Lomonosov Moscow State University / Depament of General Physics. The interest to functional and structural indicators of mycobiota with a respective to use them in biogiagnostics is currently rising because fungi represent as essential component among environmental decomposers of organic material in ecosystems. The complexity of utilization of fungi in biogiagnostics is explained by the variability of reactions to external stimuli due to their physiological and biochemical plasticity. In this regard, it seems very important to find the reactions of fungal cultures adequately reflecting their response to external stimuli in different conditions. The essential biogenic fluorophores such as NADH, tryptophan, melanin, ergosterol, pyridoxine, riboflavin, FAD, and FMN can be monitored instrumentally by spectroscopic techniques. The aim of the present study was to investigate the features of fluorescence spectra of fulamentous fungi cultivated under different concentrations of source of bioavailable and nor readily bioavailable carbon in the growth medium. The research objects were strains of Alternaria alternata, Cladosporium cladosporioides, and Trichoderma harzianum. The strains were kindly provided by O.E. Marfenina and A.E. Ivaniva, Soil Science Faculty of MSU. The filamentous fungi were grown on liquid and agar Czapek medium contained a varying level of sucrose (0.3 and 3%) and humic substances (0.02 and 0.1%). Fluorescence spectra were measured using a luminescence spectrometer Solar CM2203 at several wavelengths of the exciting radiation (250, 280, 310, 350, and 450 nm). Fluorescence excitation spectra were registered for emission at 350, 440, and 460 nm. Typical fluorescence spectra of fungal samples (spore suspensions and supernatant liquids) with the UV-excitation consist of two overlapping bands. The UV-band with maximum at 300-350 nm under excitation at 280 nm is a protein-like fluorescence, and the band in the blue region with the maximum at 400-450 nm under excitation at 310-370 nm is emission of fungal chromophores like NADH and or melanins. We suggest using the intensity of the first fluorescence band in the fungal samples for rapid evaluation of spore biomass. Measured fluorescence characteristics were found correlating with saturation of growth medium by source of bioavailable and not readily bioavailable carbon. Therefore we consider this research as promising on the way of using fungal fluorophores to assess responses of filamentous fungi to external stimuli.

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