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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Signaling and Adaptation Modulate the Dynamics of the Photosensoric Complex of Natronomonas pharaonisстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Orekhov PS, Klose D., Mulkidjanian AY, Shaitan KV, Engelhard M., Klare JP, Steinhoff HJ
- Журнал: PLoS Computational Biology
- Том: 11
- Номер: 10
- Год издания: 2015
- Издательство: Public Library of Science
- Местоположение издательства: United States
- DOI: 10.1371/journal.pcbi.1004561
- Аннотация: Motile bacteria and archaea respond to chemical and physical stimuli seeking optimal conditions for survival. To this end transmembrane chemo- and photoreceptors organized in large arrays initiate signaling cascades and ultimately regulate the rotation of flagellar motors. To unravel the molecular mechanism of signaling in an archaeal phototaxis complex we performed coarse-grained molecular dynamics simulations of a trimer of receptor/transducer dimers, namely NpSRII/NpHtrII from Natronomonas pharaonis. Signaling is regulated by a reversible methylation mechanism called adaptation, which also influences the level of basal receptor activation. Mimicking two extreme methylation states in our simulations we found conformational changes for the transmembrane region of NpSRII/NpHtrII which resemble experimentally observed light-induced changes. Further downstream in the cytoplasmic domain of the transducer the signal propagates via distinct changes in the dynamics of HAMP1, HAMP2, the adaptation domain and the binding region for the kinase CheA, where conformational rearrangements were found to be subtle. Overall these observations suggest a signaling mechanism based on dynamic allostery resembling models previously proposed for E. coli chemoreceptors, indicating similar properties of signal transduction for archaeal photoreceptors and bacterial chemoreceptors.
- Добавил в систему: Фенюк Борис Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | пдф | plos_comp_biol_2015.pdf | 4,0 МБ | 16 июня 2017 [ShaitanKV] |