Activation/inactivation role of ionic liquids on formate dehydrogenase from Pseudomonas sp. 101 and its mutated thermostable form

Авторы: D’Oronzo E., Secundo F., Minofar B., Kulik N., Pometun A.A., Tishkov V.I.
Журнал: ChemCatChem
Том: 10
Год издания: 2018
Издательство: WILEY-VCH
Местоположение издательства: INC, 605 THIRD AVE, NEW YORK, USA, NY, 10158-0012
DOI: 10.1002/cctc.201800145
Аннотация: Ionic liquids are used in numerous research areas including biocatalysis. The effect of ILs/water mixture on the activity of wild type and a more thermally and chemically stable mutant (SM4) of a specific formate dehydrogenases (PseFDH, EC 1.2.1.2) were studied experimentally and by molecular dynamics (MD) simulations. The ILs investigated were [Mmim][Me2PO4], [Bmim][Br], [Bmim][CH3SO3], [Bmim][BF4], [Bmim][AcO] and it was found that low concentrations (optimally 2.5 %) of some ILs increased (up to 42 %) the activity of the SM4 FDH but not for the WT FDH. Using intrinsic fluorescence to calculate Stern-Volmer constants and thermodynamic parameters, we have studied protein conformational changes due to ILs for both enzymes. Kinetic analyses allowed to shed light on the mechanism of activation by 2.5 % [Bmim][BF4] on the mutant enzyme. MD simulation provided evidences of a molecular basis of different enzyme activities in ILs that well correlated with the experimental data observed
Добавил в систему: Тишков Владимир Иванович

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