3D-Scaffolds from Poly(3-hydroxybutyrate)Poly(ethylene glycol) Copolymer for Tissue Engineering

Bonartsev, A.P.; Zharkova, I.I.; Yakovlev, S.G.; Myshkina, V.L.; Makhina, T.K.; Zernov, A.L.; Kudryashova, K.S.; Feofanov, A.V.; Akulina, E.A.; Ivanova, E.V.; Zhuikov, V.A.; Andreeva, N.V.; Voinova, V.V.; Bessonov, I.V.; Kopitsyna, M.V.; Morozov, A.S.; Bonartseva, G.A.; Shaitan, K.V.; Kirpichnikov, M.P.

Авторы: Bonartsev A.P., Zharkova I.I., Yakovlev S.G., Myshkina V.L., Makhina T.K., Zernov A.L., Kudryashova K.S., Feofanov A.V., Akulina E.A., Ivanova E.V., Zhuikov V.A., Andreeva N.V., Voinova V.V., Bessonov I.V., Kopitsyna M.V., Morozov A.S., Bonartseva G.A., Shaitan K.V., Kirpichnikov M.P.
Журнал: Journal of Biomaterials and Tissue Engineering
Том: 6
Номер: 1
Год издания: 2016
Первая страница: 42
Последняя страница: 52
DOI: 10.1166/jbt.2016.1414
Аннотация: Poly(3-hydroxybutyrate)-poly(ethylene glycol) (PHB-PEG) copolymer is a novel member of polyhydroxyalkanoates (PHAs) produced by biotechnological PEGylation with improved biocompatibility and biodegradability. We used the PHB-PEG to produce the porous 3D scaffolds for bone tissue engineering. The PHB-PEG scaffolds were made by gas porous formation technique using ammonium carbonate as a porogen. The 3D-scaffolds on the base of homopolymer poly(3-hydroxybutyrate) (PHB) and its blend with PEG 70:30 w/w (PHB+PEG) were used for comparison. In this study morphology (e.g. porosity), hydrophilicity, thermal properties and protein adsorption of PHB-PEG 3D scaffolds were examined. Bone marrow stromal cells (BMSCs) and 3T3 fibroblast cells were cultured on PHB-PEG 3D scaffolds. Cell viability, growth and adhesion on polymer 3D scaffolds were investigated by XTT test and scanning electron microscopy. The obtained data showed that the PHB-PEG copolymer scaffolds demonstrated lower hydrophobicity and better biocompatibility in comparison with the PHB homopolymer scaffolds and these indicators were not inferior to the PHB+PEG blend. It was also shown that PHB-PEG 3D scaffolds are suitable substrate for cell growth and could be applied for bone tissue engineering.
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