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Preparation of growth hormone receptor GHR-(254–298) transmembrane fragments in a cell-free expression system for structural studiesстатья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 января 2016 г.
- Авторы: Bocharova O.V., Kuzmichev P.K., Urban A.S., Goncharuk S.A., Bocharov E.V., Arsenyev A.S.
- Журнал: Russian Journal of Bioorganic Chemistry
- Том: 41
- Номер: 6
- Год издания: 2015
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 631
- Последняя страница: 637
- DOI: 10.1134/S1068162015060047
- Аннотация: Growth hormone somatotropin and its membrane receptor GHR (growth hormone receptor) belonging to a superfamily of the type I receptors with tyrosine kinase activity are involved in the intercellular signal transduction cascade that regulates a number of important physiological and pathological processes in humans. Binding with somatotropin triggers a transition of GHR between two alternative dimer states, resulting in an allosteric activation of JAK2 (Janus kinase 2) tyrosine kinase in the cell cytoplasm. Transmembrane domain of GHR is directly involved in this complex conformational transition. It has presumably two dimerization interfaces corresponding to the “unliganded” and the active state of GHR. In order to study the molecular basis of biochemical signal transduction mechanism across the cell membrane, we have developed an efficient cell-free production system of a TM fragment of GHR, which contains its TM domain flanked by functionally important juxtamembrane regions (the GHRtm fragment, residues 254–298). The developed system allows obtaining ~1 mg per 1 mL of reaction mixture of 13C15N- and 15N-isotope-labeled protein for structural and dynamic studies of the GHR TM domain dimerization in the membrane-mimicking medium by high-resolution heteronuclear NMR spectroscopy.
- Добавил в систему: Гончарук Сергей Александрович