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The Prokaryotic Community of Subglacial Bottom Sediments of Antarctic Lake Untersee: Detection by Cultural and Direct Microscopic Techniquesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 13 февраля 2018 г.
- Авторы: Mulyukin A.L., Demkina E.V., Manucharova N.A., Akimov V.N., Andersen D., McKay C., Gal’chenko V.F.
- Журнал: Microbiology
- Том: 83
- Номер: 1-2
- Год издания: 2014
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 77
- Последняя страница: 84
- Оригинальные версии: ПРОКАРИОТНОЕ СООБЩЕСТВО ПОДЛЕДНИКОВЫХ ГРУНТОВ АНТАРКТИЧЕСКОГО ОЗЕРА УНТЕРЗЕЕ: ВЫЯВЛЕНИЕ КУЛЬТУРАЛЬНЫМИ И ПРЯМЫМИ МИКРОСКОПИЧЕСКИМИ МЕТОДАМИ” МИКРОБИОЛОГИЯ
- DOI: 10.1134/S0026261714020143
- Аннотация: The heterotrophic mesophilic microbial component was studied in microbial communities of the samples of frozen regolith collected from the glacier near Lake Untersee collected in 2011 during the joint Rus- sian–American expedition to central Dronning Maud Land (Eastern Antarctica). Cultural techniques revealed high bacterial numbers in the samples. For enumeration of viable cells, the most probable numbers (MPN) method proved more efficient than plating on agar media. Fluorescent in situ hybridization with the relevant oligonucleotide probes revealed members of the groups Eubacteria (Actinobacteria, Firmicutes) and Archaea. The application of the methods of cell resuscitation, such as the use of diluted media and prevention of oxidative stress, did not result in a significant increase in the numbers of viable cells retrieved from subglacial sediment samples. Our previous investigations demonstrated the necessity for special procedures for efficient reactivation of the cells from microbial communities of replace with buried soil and permafrost samples collected in the Arc- tic zone. The differing responses to the special resuscitation procedures may reflect the differences in the phys- iological and morphological state of bacterial cells in microbial communities subject to continuous or periodic low temperatures and dehydration. Keywords: subglacial sediments, Antarctics, prokaryotes, cell numbers, cell resuscitation, FISH
- Добавил в систему: Манучарова Наталия Александровна