|
ИСТИНА |
Войти в систему Регистрация |
Интеллектуальная Система Тематического Исследования НАукометрических данных |
||
The Effect of TNF and VEGF on the Properties of Ea.hy926 Endotheli-al Cells in a Model of Multi-Cellular Spheroidsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из перечня ВАК
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 октября 2018 г.
- Авторы: Gapizov S.Sh, Petrovskaya L.E., Shingarova L.N., Svirschevskaya E.V., Dolgikh D.A., Kirpichnikov M.P.
- Журнал: Acta Naturae (англоязычная версия)
- Том: 10
- Номер: 1
- Год издания: 2018
- Издательство: Park Media Ltd
- Местоположение издательства: Moscow, Russia (Federation)
- Первая страница: 34
- Последняя страница: 42
- Аннотация: Endothelial cells play a major role in the development of inflammation and neoangiogenesis in cancer and chronic inflammatory diseases. In 3D cultures, cells are under conditions that closely resemble those exist- ing in healthy and disease-stricken human organs and tissues. Therefore, the development of a 3D model based on the Ea.hy926 endothelial cell line is an urgent need in molecular and cellular biology. Cell cultivation on an anti-adhesive substrate under static conditions was shown to lead to the formation of spheroids (3D cultures). Expression of ICAM-1 and VEGFR-2 and production of cytokines were screened in 2D and 3D cultures in the presence of TNF and VEGF. According to flow cytometry and confocal microscopy data, TNF significantly in- creased the expression of the cell adhesion molecule ICAM-1 in both 2D and 3D cultures but did not affect the expression level of VEGFR-2. Increased production of pro-inflammatory (IL-8, IL-6, IP-10) and anti-inflam- matory (IL-10, TGF-β 1–3) factors was observed in spontaneous 3D cultures but not in 2D cultures, which was confirmed by flow cytometry and qPCR. TNF-induced secretion of IL-10, GM-CSF, and IL-6 was 11-, 4.7-, and 1.6-fold higher, respectively, in 3D cultures compared to 2D cultures. Thus, the use of a Ea.hy926 3D cell culture is a promising approach in studying the effects of anti- and pro-inflammatory agents on endothelial cells.
- Добавил в систему: Гапизов Султан Шахбанович