Photo-induced pH changes in the vicinity of isolated Peperomia metallica chloroplastsстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Photo-induced pH changes of the external medium in a region immediately adjacent to the surface of individual isolated Peperomia metallica chloroplasts have been measured using antimony pH-micro-electrodcs. The pH changes induced by continuous illumination were composed of two phases: an initial alkalization (phase I) and a subsequent acidification (phase II) of the medium. Both phases were severely suppressed by DCMU and protonophorous uncouplers but they showed different sensitivity towards DCCD, NH4Cl and some other agents. Phase I was selectively inhibited by DCCD and was partially restored upon addition of ATP to DCCD-poisoned chloroplasts. Phase II was inhibited by 1.0 mol/m3 NH4Cl. It appears from these data that phases I and II of light-induced pH changes are determined by different processes. It is suggested that the pH increase (phase I) is due to a photosynthetic CO2 fixation and the pH decrease (phase II) is caused by a light-dependent extrusion of protons from intact chloroplasts.