Promoter-specific regulation of PPARGC1A gene expression in human skeletal muscleстатья

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Дата последнего поиска статьи во внешних источниках: 11 января 2016 г.

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[1] Promoter-specific regulation of ppargc1a gene expression in human skeletal muscle / D. Popov, E. Lysenko, T. Vepkhvadze et al. // Journal of Molecular Endocrinology. — 2015. — Vol. 55, no. 2. — P. 159–168. The goal of this study was to identify unknown transcription start sites of the PPARGC1A (PGC-1α) gene in human skeletal muscle and investigate the promoter-specific regulation of PGC-1α gene expression in human skeletal muscle. Ten amateur endurance-trained athletes performed high- and low-intensity exercise sessions (70 min, 70% or 50% V'O2max). High-throughput RNA sequencing and exon–exon junction mapping were applied to analyse muscle samples obtained at rest and after exercise. PGC-1α promoter-specific expression and activation of regulators of PGC-1α gene expression (AMPK, p38 MAPK, CaMKII, PKA and CREB1) after exercise were evaluated using qPCR and western blot. Our study has demonstrated that during post-exercise recovery, human skeletal muscle expresses the PGC-1α gene via two promoters only. As previously described, the additional exon 7a that contains a stop codon was found in all samples. Importantly, only minor levels of other splice site variants were found (and not in all samples). Constitutive expression PGC-1α gene occurs via the canonical promoter, independent of exercise intensity and exercise-induced increase of AMPKThr172 phosphorylation level. Expression of PGC-1α gene via the alternative promoter is increased of two orders after exercise. This post-exercise expression is highly dependent on the intensity of exercise. There is an apparent association between expression via the alternative promoter and activation of CREB1. [ DOI ]

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