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Quantitative Lateral Flow Immunoassay for Total Prostate Specific Antigen in Serumстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 апреля 2016 г.
- Авторы: Andreeva I.P., Grigorenko V.G., Egorov A.M., Osipov A.P.
- Журнал: Analytical Letters
- Том: 49
- Номер: 4
- Год издания: 2016
- Издательство: Marcel Dekker Inc.
- Местоположение издательства: United States
- Первая страница: 579
- Последняя страница: 588
- DOI: 10.1080/00032719.2015.1075130
- Аннотация: A simple method for the rapid determination of prostate-specific antigen (PSA) in serum is reported using a lateral flow immunoassay with gold nanoparticles as the label. The method uses the intensity of colored test lines to determine PSA from 0.3 to 30 ng/ml. The limit of detection was 0.3 ng/ml and the coefficient of variation was less than 10%. The analysis time was approximately twenty minutes. The novel method showed good correlation with enzyme linked immunosorbent assay (ELISA) measurements of prostate-specific antigen concentration in human serum with a linear regression coefficient of 0.985. The developed system was stable for at least twelve months when stored from +4 to 30°C and has potential application for clinical practice.
- Добавил в систему: Осипов Александр Павлович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | andreeva2015.pdf | 788,3 КБ | 14 сентября 2017 [APOsipov] |
Библиографическая ссылка на публикацию в стиле
ГОСТ |
plain |
abbrv |
acm |
alpha |
amsalpha |
amsplain |
apalike |
ieeetr |
siam
| [1] | Quantitative lateral flow immunoassay for total prostate specific antigen in serum / I. P. Andreeva, V. G. Grigorenko, A. M. Egorov, A. P. Osipov // Analytical Letters. — 2016. — Vol. 49, no. 4. — P. 579–588. A simple method for the rapid determination of prostate-specific antigen (PSA) in serum is reported using a lateral flow immunoassay with gold nanoparticles as the label. The method uses the intensity of colored test lines to determine PSA from 0.3 to 30 ng/ml. The limit of detection was 0.3 ng/ml and the coefficient of variation was less than 10%. The analysis time was approximately twenty minutes. The novel method showed good correlation with enzyme linked immunosorbent assay (ELISA) measurements of prostate-specific antigen concentration in human serum with a linear regression coefficient of 0.985. The developed system was stable for at least twelve months when stored from +4 to 30oC and has potential application for clinical practice. [ DOI ] |