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Detection of anaerobic sulfate-reducing bacteria in oxygen-containing upper water layers of the Black and Baltic seasстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 5 августа 2018 г.
- Авторы: Bryukhanov A.L., Korneeva V.A., Pimenov N.V.
- Журнал: Moscow University Biological Sciences Bulletin
- Том: 70
- Номер: 4
- Год издания: 2015
- Издательство: Allerton Press
- Местоположение издательства: New York, N.Y., United States
- Первая страница: 184
- Последняя страница: 188
- DOI: 10.3103/S0096392515040057
- Аннотация: Fluorescent in situ hybridization (FISH) and PCR were used for analysis of phylogenetic structure of anaerobic sulfate-reducing bacterial communities in oxygen-containing upper water layers of meromictic basins – the Black Sea and the Gdansk Deep of the Baltic Sea. In the Black Sea (continental slope at depths 30-70 m), cells of sulfate-reducing bacteria (SRB) hybridizing with 16S rRNA-specific FISH-probes for Desulfotomaculum, Desulfobacter, and Desulfovibrio genera were revealed, whereas Desulfomicrobium-related bacteria were prevalent in the chemocline zone at a 150-m depth. Besides Desulfotomaculum (SRB subgroup 1), Desulfobacter (SRB subgroup 4), and Desulfovibrio-Desulfomicrobium (SRB subgroup 6), nested PCR with the use of 16S rRNA gene-specific primers detected the presence of Desulfococcus-Desulfonema-Desulfosarcina (SRB subgroup 5) in the oxygen-containing water column of the Black and Baltic seas. Active enrichment SRB culture that contained bacterium Desulfosporosinus sp. as a major component was obtained from the Black Sea water sample collected at a 70-m depth.
- Добавил в систему: Брюханов Андрей Леонидович