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Electrosynthesis and binding properties of molecularly imprinted poly-o-phenylenediamine as artificial antibodies for electroanalysis of myoglobinстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 декабря 2015 г.
- Авторы: Shumyantseva V.V., Bulko T.V., Sigolaeva L.V., Kuzikov A.V., Shatskaya M.A., Archakov A.I.
- Журнал: Doklady Biochemistry and Biophysics
- Том: 464
- Номер: 1
- Год издания: 2015
- Издательство: M A I K Nauka - Interperiodica
- Местоположение издательства: Russian Federation
- Первая страница: 275
- Последняя страница: 278
- DOI: 10.1134/S1607672915050038
- Аннотация: Molecularly imprinted poly-o-phenylenediamine with template myoglobin molecules (i.e., polymeric antibodies to myoglobin, molecularly imprinted polymer, MIP) was synthesized via electropolymerization. Electropolymerization, washing, and the interaction of the polymeric antibodies with myoglobin was examined by square wave voltammetry and microgravimetry. The analysis of myoglobin was carried out through direct electrochemical detection of the reduction peak of Fe3+ of the hemeprotein on screen-printed graphite electrodes modified by the MIP. According to the electrochemical analysis, MIP surfaces demonstrated remarkably higher ability to bind the protein compared to that of surfaces prepared by the same route under the same conditions but in the absence of myoglobin (surfaces of the non-imprinted polymer, NIP). The imprinting factor Imax(MIP)/Imax(NIP) was found to be 2–4. The equilibrium dissociation constant Kd of the interaction of myoglobin with MIP electrodes was evaluated as (2.4 ± 0.5) × 1E–08 M. The lower detection limit of myoglobin by a MIP sensor was determined as 0.5E0–09 M, the range of detectable concentrations being 1E–09–1E–05 M.
- Добавил в систему: Сиголаева Лариса Викторовна