Immobilization of microalgae on the surface of new cross-linked polyethylenimine-based sorbentsстатья

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Дата последнего поиска статьи во внешних источниках: 14 февраля 2019 г.

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1. manuscript_printed.pdf manuscript_printed.pdf 2,5 МБ 18 сентября 2018 [VasilievaSvetlana]

[1] Immobilization of microalgae on the surface of new cross-linked polyethylenimine-based sorbents / S. Vasilieva, K. Shibzukhova, A. Morozov et al. // Journal of Biotechnology. — 2018. — Vol. 281. — P. 31–38. We report on the use of the polyethylenimine-based (PEI) sorbents for immobilization and harvesting of microalgae (MA) cells. Specific materials assessed were porous solid polymers from highly-branched PEI synthesized by cross-linking with epichlorohydrin (ECH) or diethylene glycol diglycidyl ether (DGDE). We estimated the effect of PEI/cross-linker ratio on the MA attachment and biocompatibility of the sorbents with the MA cells. A decrease in the cross-linker percentage resulted in the enhancement of the immobilization efficiency but impaired the cell viability as was manifested by inhibition of the photosynthetic activity of the MA cells. The rate of Chlorella vulgaris cell attachment to the sorbents with ECH was faster as compared to that of the PEI–DGDE-based polymers. The cells immobilized on the PEI–ECH sorbents showed a more profound decline in their viability (assessed via photosynthetic activity). The sorbents with 60% of DGDE were characterized by a high immobilization efficiency. These sorbents supported a prolonged cultivation of the immobilized MA without impairing their viability and metabolic activity. We conclude that the sorbents with a lower percentage of DGDE (< 30%) and sorbents with ECH are suitable for harvesting of the MA cells intended for immediate downstream processing, potentially without the cell desorption. To the best of our knowledge, this is the first report on successful application of PEI-based sorbents in microalgal biotechnology. [ DOI ]

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