Inhibition of Mn(2+)-induced error-prone DNA synthesis with Cd(2+) and Zn(2+)

Gening, L.; Lakhin, A.; Stelmashook, E.; Isaev, N.; Tarantul, V.

Авторы: Gening LV, Lakhin AV, Stelmashook EV, Isaev NK, Tarantul VZ
Журнал: Biochemistry (Moscow)
Том: 78
Номер: 10
Год издания: 2013
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 1137
Последняя страница: 1145
DOI: 10.1134/S0006297913100088
Аннотация: Bivalent metal cations are key components in the reaction of DNA synthesis. They are necessary for all DNA polymerases, being involved as cofactors in catalytic mechanisms of nucleotide polymerization. It is also known that in the presence of Mn2+ the accuracy of DNA synthesis is considerably decreased. The findings of this work show that Cd2+ and Zn2+ selectively inhibit the Mn2+-induced error-prone DNA polymerase activity in extracts of cells from human and mouse tissues. Moreover, these cations in low concentrations also can efficiently inhibit the activity of homogeneous preparations of DNA polymerase iota (Pol ι), which is mainly responsible for the Mn2+-induced error-prone DNA polymerase activity in cell extracts. Using a primary culture of granular cells from postnatal rat cerebellum, we show that low concentrations of Cd2+ significantly increase cell survival in the presence of toxic Mn2+ doses. Thus, we have shown that in some cases low concentrations of Cd2+ can display a positive influence on cells, whereas it is widely acknowledged that this metal is not a necessary microelement and is toxic for organisms.
Добавил в систему: Исаев Николай Константинович

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

Inhibition of Mn(2+)-induced error-prone DNA synthesis with Cd(2+) and Zn(2+)статья