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Rapid flow-through enzyme immunoassay of progesterone in whole cows' milkстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 февраля 2021 г.
- Авторы: Samsonova J.V., Safronova V.A., Osipov A.P.
- Журнал: Analytical Biochemistry
- Том: 545
- Год издания: 2018
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 43
- Последняя страница: 48
- DOI: 10.1016/j.ab.2018.01.011
- Аннотация: A rapid flow-through immunoassay using an enzyme (horseradish peroxidase) as a label for quantitative and semi-quantitative determination of progesterone in whole cows` milk was developed. The flow-through test device consisted of a porous nitrocellulose membrane coated with antibodies and an absorbent membrane. The substrate solution containing 3,3′,5,5′ - tetramethylbenzidine was used for colour visualization. The detection limit of 0.4 ng/mL P4 was obtained by this method; analysis time did not exceed 15 minutes. To eliminate matrix interference a simple sample preparation procedure was used. Results of analysis of whole cows’ milk samples with flow-through method were in good correlation with ELISA results (R=0.96,n=34). The developed rapid flow-through test system showed high efficiency for the determination of progesterone level in whole cow's milk and can be used on-site for quick identification of milk samples with low and high progesterone concentration.
- Добавил в систему: Осипов Александр Павлович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 1-s2.0-S0003269718300204-main.pdf | 1-s2.0-S0003269718300204-main.pdf | 512,9 КБ | 12 марта 2018 [jvs] |