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Aspects of interaction of putative anchorosomal protein p68 with the nuclear envelope during the cell cycleстатья
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Kurchashova S.Yu, Prusov A.N., Gulak P.V., Kireev I.I., Polyakov V.Yu
- Журнал: Russian Journal of Developmental Biology
- Том: 31
- Номер: 6
- Год издания: 2000
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 362
- Последняя страница: 371
- Оригинальные версии: Особенности взаимодействия белка анкоросом р68 с ядерной оболочкой в клеточном цикле
- Аннотация: In the interphase nucleus, the chromatin associated with the nuclear envelope is represented by a layer of anchorosomes, granules with a diameter of 20–25 nm. Biochemically, the fraction of chromatin directly associated with the nuclear envelope is characterized by resistance against decondensing influences, a low level of DNA methylation, and presence of specific acid-soluble proteins. However, the mechanisms lying at the base of chromatin-nuclear envelope interaction have been insufficiently studied. Specifically, it is unknown whether anchorosomes are constant structures or subject to reversible disassembly, when the contacts between chromatin and nuclear envelope are destroyed. We obtained immune serum recognizing a 68 kDa protein from the nuclear envelopes fraction and studied the localization of this protein in interphase and mitotic cells. We show that this protein, present in the NE/anchorosomal fraction, does not remain bound with chromosomes during mitosis. It dissociates from chromosomes at the beginning of the prophase and then can be identified again at the periphery of the newly forming nuclei in the telophase.
- Добавил в систему: Киреев Игорь Игоревич