Организация, в которой проходила защита:
Сколковский институт науки и технологий
Год защиты:2023
Аннотация:Genome topology plays a crucial role in regulation of gene expression programs as it both enables enhancer-promoter interactions and contributes to isolation of active regions to constrain the spread of enhancers influence. Abnormal genome spatial organization leads to impaired regulation of gene expression programs and, thus, disease progression. The large number of epidermis’ diseases such as dermatitis, ichthyosis, psoriasis and epidermoid carcinomas determined the importance of studying genome topology and regulation in keratinocytes.
In our research, we investigated spatial organization of the human keratin gene locus in cell lines with active and repressed keratin expression. We found out that the locus topology is cell-type specific and mostly relies on spatial networks between potential regulatory elements. We also studied the binding profile of architectural protein CTCF as well as transcriptional factor p63 in putative locus control regions and demonstrated that CTCF binding is not correlated with keratin gene expression while p63 binding is associated with the locus activity.
