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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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We present a compact laser optical toolbox integrated with a dual-channel 2PEF microscopy for the investigation of nonlinear optical properties of fluorescent indicators and its potential for ratiometric two-photon imaging. We provided accurate nonlinear transformation of low power pulses in a photonic crystal fiber (PCF) to an ultrashort sub-octave-spanning supercontinuum (SC) generation and its amplitude and phase tuning to produce an appropriate source for both TPA spectra measurements and ratiometric dual-channel 2PEF imaging.