ИСТИНА

In this work, silicon nanoparticle ensembles fabricated through electrochemical etching (porous silicon) followed by picosecond laser ablation (1064 nm, 34 ps, 10 mJ, 10 Hz) in water were studied. The optical characteristics of investigated object were retrieved from measured spectra of collimated and diffuse transmittance and diffuse reflectance in the wavelength range of 400-1100 nm applying the original technique, based on least-scattering approximation and the Beer–Lambert–Bouguer law. All measurements were obtained using the AnalytikJena Specord 250 Plus spectrophotometer equipped with an integrating sphere. The potential of silicon nanoparticles for contrasting structural elements in bioimaging applications was studied in ex vivo experiment on controlling optical properties of murine biotissues with nanoparticle administration. The experiments were performed with brain and hind limb muscle tissue samples extracted from 4 laboratory mice and subsequently homogenized. Two samples were prepared for each tissue types: i) composition of homogenized tissue and phosphate buffered saline ii) composition of homogenized tissue and silicon nanoparticle suspensions The ratio of concentrations was considered 1:1 for both samples. Additionally, the potential of silicon nanoparticles for contrasting structural elements in OCT diagnostics were studied through Monte Carlo simulations.