ИСТИНА

Transformation using plant cell cultures and isolated tissues is commonly employed to obtain genetically modified plants. The selection of optimal cultivation conditions at different stages, including in vitro culture obtainment, induction of morphogenesis, and regeneration of whole plants, is critical for successful plant transformation. We evaluated the callus formation ability and regenerative capacity of 10 varieties of Fagopyrum esculentum and Fagopyrum tataricum and selected the most promising ones for regenerated plant production: Batyr, Shinano, and KK8. The optimal medium for callus formation contained 2 mg/L 2,4-Dichlorophenoxyacetic acid and 2 mg/L Kinetin. The medium for plant regeneration contained 6-Benzylaminopurine (2 mg/L), Kinetin (0.2 mg/L), and Indole-3-acetic acid (0.2 mg/L). Regenerated plants of the Batyr variety were successfully acclimated to non-sterile soil conditions. This protocol can be used for the genetic transformation of buckwheat in future studies. Supported by the Ministry of Science and Higher Education, project #075-15-2021-1064