ИСТИНА

Nanosized polymer films can be constructed via alternative electrostatic adsorption of polyelectrolytes. The adsorption is to a great extent determined by the state and structure of initial surface and/or the pre-adsorbed layers. The same rule is apparently valid for electrostatic adsorption of enzymes used for their immobilization. This is specifically important for a design and fabrication of bioanalytical devices, e.g., biosensors, where the amount of an enzyme incorporated and the strength of its binding influence on basic characteristics, such as activity and operation stability, of these bioanalytical surfaces. To demonstrate this, different fabrication regimes and properties of polymer/enzyme thin films adsorbed onto conductive substrates were examined. The films were formed via two-steps, sequential adsorption of a polymeric component followed by the enzyme adsorption under different pH/salt composition and temperature regimes. Strong/weak linear polyelectrolytes, amphiphilic ionogenic diblock copolymers or microgels were considered as polymeric components to be interacted with enzymes. Specific applications to the field of biosensors are considered. (p. 30)