ИСТИНА

At the moment there is strong evidence for the antioxidant properties ofhydrogen, which makes it prospective to study hydrogen effects on such an experiment model asmonocrotaline-induced pulmonary hypertension (MCT-PH), the pathogenesis of which isassociated with oxidative stress and inflammation.OBJECTIVES: The aim of the work was to investigate the influence of 4% hydrogen in the airon markers of 3-week MCT-PH development in male rats.METHODS: For modeling MCT-PH we used a single subcutaneous injection of monocrotalinealcohol solution (60 mg/kg, Sigma Aldrich) to male Wistar rats (MCT-group, n=16). Controlanimals were injected with solvent only (Control group, n=8). Then the MCT-group was dividedinto two groups: MCT-Control rats (n=8) were kept in boxes perfused with room air, and MCT-H 2 rats, were kept in a box perfused with air containing 4% hydrogen for three weeks. After 21days, right ventricular systolic pressure (RVSP) and mean systemic pressure (MSP) weremeasured in all animals under urethane anesthesia. In addition, right ventricular hypertrophy(RVH) was assessed and histological analysis of lung tissue was performed, in which the numberand quality of mast cells (MC) were evaluated.RESULTS: There was an average increase of 20 mm Hg in the MCT group compared to theControl-group (37±5 mm Hg), indicating the development of MCT-PH. No differences werefound between the MCT groups. However, there was a significant decrease of MSP value (82±7mm Hg) in MKT-H2 group compared to K group (93±9 mm Hg) and MCT-Control group (95±7mm Hg) (p<0.05). Histological analysis showed that the number of TCs including thosecontaining tryptases in the group, MCT-H2 was on average 50% lower than in the MCT-Controlgroup.CONCLUSIONS: Breathing a mixture of air containing 4% hydrogen for 3 weeks afterinitiation of MCT-PH had no effect on the degree of MCT-PH development, but resulted in adecrease in MSP, which was accompanied by a decrease in signs of inflammation in the lungs.