ИСТИНА

Previously, the effective use of carbocyanine dyes for the visualization of "small molecules" by using non-covalent interactions was studied. We demonstrated the possibility of determining aminoglycosides using the strategy of enhancing the fluorescence of carbocyanines in surfactant solutions at premicellar concentrations [1, 2]. In continuation of these studies, a convenient method for monitoring the delivery of large hydrophilic molecules into cells based on the use of chitosan nanocontainers has been developed. A series of pentamethine carbocyanines containing hydrophobic substituents has been synthesized. Carbocyanine 1 turned out to be the most promising for solving this problem. Using this dye, a method for imaging-guided drug delivery to cells was developed and an approach was proposed for visualizing drug release in the organism.