ИСТИНА

The imbalance between the production of reactive oxygen species (ROS) and the cell's ability to neutralize them is called oxidative stress. In the sperm, this process can lead to lipid peroxidation, DNA fragmentation, changes in motility, and, as a result, infertility due to the incompleteness of the antioxidant system of the cell. Antioxidants can slow down or stop oxidative stress by engaging in redox reactions with ROS. Melatonin and glutathione are endogenous antioxidants that are present in human metabolism and have their own chain of reactions. From a practical point of view, the use of antioxidants to prevent oxidative stress in sperm may provide better results in cryopreservation and in vitro fertilization. As a result of the experiment, it was found that Melatonin significantly reduces the degree of DNA fragmentation during the induction of oxidative stress. By spectrophotometry, the concentration of hydrogen peroxide during the induction of endogenous oxidative stress was determined, which was C𝐻2𝑂2 = 6.3 ± 0.5 μ𝑀. Also, melatonin is not toxic and can be recommended as an effective antioxidant for working with cell cultures.