ИСТИНА |
Войти в систему Регистрация |
|
Интеллектуальная Система Тематического Исследования НАукометрических данных |
||
The measurement of the kinetics of chlorophyll fluorescence transient of algae and plants is a widely used method for determining the state of photosynthetic apparatus. The time dependence of the fluorescence intensity is a complex multiphase curve, which depicts the various stages of the electron transfer in the electron transport chain of chloroplasts. We proposed the method of spectral multi-exponential approximation (SMEA) which allows to formalize phase identification, to estimate quantitative characteristics of individual phases of the induction curve (amplitudes and characteristic times), and to reveal hidden phases of the curve. Occurrence of additional phases on the induction curve points to a change in the functioning of the photosynthetic apparatus in response to changing growth conditions. The proposed method is implemented in pyPhotoSyn software, which allows carrying out a comprehensive analysis of large sets of experimental data. This allows monitoring of evolution of fluorescence transient curves in various growth conditions. In this work we used SMEA to study the fluorescence transient data measured for microalgae (Chlorella sp., Chlamidimonas reinhardtii) cultivated under nitrogen or sulfur deprivation. In both cases of mineral starvation we observed the appearance of an additional phase in microseconds range. We suppose that occurrence of this phase points to the processes emerging in response to starvation. A high sensitivity of SMEA allows to suggest it for detection of early cell response to stress. This work was supported by RFBR grant № 14-04-00326-a.