ИСТИНА

Initiation of protein synthesis is ruled by three canonical factors, IF1, IF2 and IF3. They were studied in detail in bacteria, where they play essential role in the recruitment of the ribosome on the mRNA, correct selection and positioning of the start codon and initiator tRNA. This system persists in human mitochondria, however it functions in rather different way due to specialization and adaptation to the organellar micro-environment. We focused on human mitochondrial IF3 which was earlier studied in vitro, but no knock-out models up to date have been published. In this work we generated the human HeLa cell lines deficient in MTIF3 gene and analyzed their mitochondrial translation