ИСТИНА

Fish egg membrane have rather complicated structure and play an important role in embryo development protecting it from external effects. Egg membrane has pores with the diameter of 0.2-0.3 μm in zebra fish (Hart and Donnovan, 1983). The nanoparticles penetration through the non-injured fish egg membrane stayes a matter of dispute up to date. The penetration of silver nanoparticles through zebra fish egg membrane at the stage of perivitelline space formation was studied in the present work. The nanoparticles were stabilized with Ampholak 7TX- tallowamphopolycarboxyglycinate. The nanoparticles size is 10 nm. The preliminary experiments demonstrated that silver nanoparticles at the concentration of 0.1 mg/l didn’t cause egg death of morphological pathology (OECD no 236). Artificial fertilization was performed in standard media, either with or without silver nanoparticles. 20 minutes later the eggs were transferred into clean media and incubated in accordance with OECD no 236. Egg death or morphological pathologies in the embryos weren’t established in the control. In test group the egg death wasn’t established but the morphological pathologies frequency was 40.5%. Besides, the considerable number of embryos didn’t hatch and possessed morphological pathologies. In the experiment with the eggs which were placed into the nanoparticles solution after perivitelline space formation and stayed into the solution for 96 hours the morphological pathologies were not found. The embryo hatching elapsed normally. The derived data allow to assume that the stage of perivitelline space formation is critical for the nanoparticles penetration through fish egg membrane.